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Methodologies - Huben's Wiki

Methodologies

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Line 37: Line 37:
 
: Non-fogging methods.
 
: Non-fogging methods.
  
;Kerosine Flotation
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==Kerosine Flotation==
: The kerosine flotation trick is widely used by acarologists and entomologists with dirty samples.  Take a sample in water, salt water, alcohol, or whatever that has a lot of soil or other heavy gunk in it.  Add a bit of kerosine, and shake the sample gently.  Kerosine wets (sticks to) chitin.  Let the kerosine float to the top with the specimens, then decant the kerosine and specimens through a mesh filter.  I have filter pans with fine stainless steel mesh, but you could use disposable paint filters.  Wash the sample with absolute alcohol to dissolve off the kerosine, then enjoy your specimens.
+
The kerosine flotation trick is widely used by acarologists and entomologists with dirty samples.  Take a sample in water, salt water, alcohol, or whatever that has a lot of soil or other heavy gunk in it.  Add a bit of kerosine, and shake the sample gently.  Kerosine wets (sticks to) chitin.  Let the kerosine float to the top with the specimens, then decant the kerosine and specimens through a mesh filter.  I have filter pans with fine stainless steel mesh, but you could use disposable paint filters.  Wash the sample with absolute alcohol to dissolve off the kerosine, then enjoy your specimens.
 +
 
 +
; [https://era.library.ualberta.ca/items/3851e3a1-10c8-4875-a2c6-0ab63ea7b876/download/2f6ab57e-d9ff-4a70-ab4c-eba60f1b7977 Kerosene floatation extraction protocol]
 +
: pages 131-6.  Appendix III in  "Biodiversity of soil arthropods in a native grassland in Alberta, Canada: obscure associations and effects of simulated climate change"

Revision as of 21:48, 31 May 2018

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