Methodologies
From Huben's Wiki
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This is a convenience page for keeping information about insect/mite collecting and preparation techniques. | This is a convenience page for keeping information about insect/mite collecting and preparation techniques. | ||
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| + | ==General== | ||
| + | ; [https://www.ars.usda.gov/ARSUserFiles/80420580/CollectingandPreservingInsectsandMites/collpres.pdf Collecting and Preserving Insects and Mites: Techniques and Tools] | ||
| + | : A classic with all the fundamentals. | ||
| + | ; [https://b-ok.lat/book/2140168/2499c9 Medical Insects and Arachnids], Lane & Crosskey, 1993. | ||
| + | : Includes basics of collecting and preserving specimens for groups with special needs such as fleas and blackflies. | ||
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| + | ==Rearing== | ||
| + | ; [https://www.amazon.com/s?k=Orin+McMonigle Orin McMonigle's large series of books on rearing invertebrates], including Orthoptera, Blattodea, Coleoptera and many non-insect arthropods. | ||
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| + | ==Pinning== | ||
| + | ; [https://www.mapress.com/zootaxa/2012/f/zt03412p061.pdf A technique to dry mount Hymenoptera (Hexapoda) from alcohol in a few seconds, and its application to other insect orders] | ||
| + | : Using a blower. | ||
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| + | ==Pointing== | ||
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| + | ; [https://www.youtube.com/watch?v=UhgW2ohlqxc&feature=youtu.be Point Mounting Small Insects on Pins] | ||
| + | : Method for beetles. | ||
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| + | I use a different method of handling and different glues: hide glue or shellac gel (because they are soluble in water and alcohol respectively.) | ||
==Pan Traps== | ==Pan Traps== | ||
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; [http://www.fao.org/fileadmin/templates/agphome/documents/Biodiversity-pollination/Pollination_Protocols/SurveyProtocolsMonitoringStatusTrendsPollinators.pdf Survey Protocols for Monitoring Status and Trends of Pollinators] | ; [http://www.fao.org/fileadmin/templates/agphome/documents/Biodiversity-pollination/Pollination_Protocols/SurveyProtocolsMonitoringStatusTrendsPollinators.pdf Survey Protocols for Monitoring Status and Trends of Pollinators] | ||
: Outline of how to use pan traps. | : Outline of how to use pan traps. | ||
| + | ; [https://www.hymenopterists.org/wp-content/uploads/2017/10/HamuliVol2Issue2.pdf The International YPT Cookbook] | ||
| + | : Lubomir Masner's advice. Find it on page 26. | ||
==Malaise Traps== | ==Malaise Traps== | ||
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; [http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0021079 The Composite Insect Trap: An Innovative Combination Trap for Biologically Diverse Sampling] | ; [http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0021079 The Composite Insect Trap: An Innovative Combination Trap for Biologically Diverse Sampling] | ||
: "The Composite Insect Trap incorporates elements from four different existing trapping methods: the cone trap, malaise trap, pan trap, and flight intercept trap." | : "The Composite Insect Trap incorporates elements from four different existing trapping methods: the cone trap, malaise trap, pan trap, and flight intercept trap." | ||
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| + | ; [https://alpineentomology.pensoft.net/article/24800/element/2/11/ An Alpine Malaise trap] | ||
| + | : Miniaturized and sticky. | ||
==Flight Intercept Traps== | ==Flight Intercept Traps== | ||
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; [https://www.dropbox.com/s/di6ngfdepqyh1ar/hamuliv9i1.pdf?dl=0A $3 Homemade Winkler Sampler] | ; [https://www.dropbox.com/s/di6ngfdepqyh1ar/hamuliv9i1.pdf?dl=0A $3 Homemade Winkler Sampler] | ||
: Hamuli VOLUME 9, ISSUE 1 — MARCH 13, 2018<br>"Beware the explorer that takes a peek below a centimeter scale, for they may find such a vast and diverse bestiary that the larger world will hold little fascination." | : Hamuli VOLUME 9, ISSUE 1 — MARCH 13, 2018<br>"Beware the explorer that takes a peek below a centimeter scale, for they may find such a vast and diverse bestiary that the larger world will hold little fascination." | ||
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| + | ==Kerosine Flotation== | ||
| + | The kerosine flotation trick is widely used by acarologists and entomologists with dirty samples. Take a sample in water, salt water, alcohol, or whatever that has a lot of soil or other heavy gunk in it. Add a bit of kerosine, and shake the sample gently. Kerosine wets (sticks to) chitin. Let the kerosine float to the top with the specimens, then decant the kerosine and specimens through a mesh filter. I have filter pans with fine stainless steel mesh, but you could use disposable paint filters. Wash the sample with absolute alcohol to dissolve off the kerosine, then enjoy your specimens. | ||
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| + | ; [https://era.library.ualberta.ca/items/3851e3a1-10c8-4875-a2c6-0ab63ea7b876/download/2f6ab57e-d9ff-4a70-ab4c-eba60f1b7977 Kerosene floatation extraction protocol] | ||
| + | : pages 131-6. Appendix III in "Biodiversity of soil arthropods in a native grassland in Alberta, Canada: obscure associations and effects of simulated climate change" | ||
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| + | ==Baited Traps== | ||
| + | ; [https://beetlesinthebush.wordpress.com/2015/12/28/beetle-collecting-101-fermenting-bait-traps-for-collecting-longhorned-beetles/ Beetle Collecting 101: Fermenting bait traps for collecting longhorned beetles] | ||
| + | : Molasses/beer and red wine baits. | ||
==Miscellaneous== | ==Miscellaneous== | ||
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; [http://baloun.entu.cas.cz/png/BassetetalRevCANART97.pdf A review of methods for sampling arthropods in tree canopies] | ; [http://baloun.entu.cas.cz/png/BassetetalRevCANART97.pdf A review of methods for sampling arthropods in tree canopies] | ||
: Non-fogging methods. | : Non-fogging methods. | ||
| − | + | ; [http://www.taxonomy.be%2Fgti_abctaxa%2Fvolumes%2Fvolume-8-manual-atbi%2Fchapter-15%2FChapter_15.pdf Sampling insects: general techniques, strategies and remarks] | |
| − | ; | + | : An excellent comparative survey of many techniques! |
| − | : | + | ; [https://www.outreachecology.com/portfolio/Samplingforestcanopybiodiversitywiththreenovelminimalcosttrapdesigns-byYDBar-NesswithMcQuillan,Whitman,Junker,Cracknell,Barrows-AusJournalofEntomology51-Feb12.pdf Sampling forest canopy arthropod biodiversity with three novel minimal-cost trap designs] |
| + | : Sticky, funnel, and flight intercept. | ||
| + | ; HDMS drying of fragile specimens | ||
| + | : We do a one-hour initial soak, change the HMDS, leave them another hour, and then drain off the HMDS and leave the samples overnight in a fume hood to dry. Doug Yanega | ||
| + | ; [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1129772/pdf/westjmed00297-0122b.pdf Water Disinfection in the Wilderness: A Simple, Effective Method of lodination] | ||
| + | : I used this for a year in Ecuador in 1987. Worked well. | ||
